Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva

Abstract [Related Publication]: Malaria is the most important vector-borne disease in the world. Epidemiological and ecological studies of malaria traditionally utilize detection of Plasmodium sporozoites in whole mosquitoes or salivary glands by microscopy or serological or molecular assays. However, these methods are labor-intensive, and can over- or underestimate mosquito transmission potential. To overcome these limitations, alternative sample types have been evaluated for the study of malaria. It was recently shown that Plasmodium could be detected in saliva expectorated on honey-soaked cards by Anopheles stephensi, providing a better estimate of transmission risk. We evaluated whether excretion of Plasmodium falciparum nucleic acid by An. stephensi correlates with expectoration of parasites in saliva, thus providing an additional sample type for estimating transmission potential. Mosquitoes were exposed to infectious blood meals containing cultured gametocytes, and excreta collected at different time points post-exposure. Saliva was collected on honey-soaked filter paper cards, and salivary glands were dissected and examined microscopically for sporozoites. Excreta and saliva samples were tested by real time polymerase chain reaction (RT-rtPCR).

The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

  • Description (parameters)
  • Plasmodium in excreta from groups of mosquitoes
  • Plasmodium in excreta, saliva and salivary glands 
  • Evaluation of parasite development in the mosquito - Parasite_dev_oocyst
  • Evaluation of parasite development in the mosquito - Parasite_dev_sporos
  • Survival analysis

The full methodology is available in the Open Access publication from the Related Publications link below.

 

    Data Record Details
    Data record related to this publication Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva
    Data Publication title Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva
  • Description

    Abstract [Related Publication]: Malaria is the most important vector-borne disease in the world. Epidemiological and ecological studies of malaria traditionally utilize detection of Plasmodium sporozoites in whole mosquitoes or salivary glands by microscopy or serological or molecular assays. However, these methods are labor-intensive, and can over- or underestimate mosquito transmission potential. To overcome these limitations, alternative sample types have been evaluated for the study of malaria. It was recently shown that Plasmodium could be detected in saliva expectorated on honey-soaked cards by Anopheles stephensi, providing a better estimate of transmission risk. We evaluated whether excretion of Plasmodium falciparum nucleic acid by An. stephensi correlates with expectoration of parasites in saliva, thus providing an additional sample type for estimating transmission potential. Mosquitoes were exposed to infectious blood meals containing cultured gametocytes, and excreta collected at different time points post-exposure. Saliva was collected on honey-soaked filter paper cards, and salivary glands were dissected and examined microscopically for sporozoites. Excreta and saliva samples were tested by real time polymerase chain reaction (RT-rtPCR).

    The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

    • Description (parameters)
    • Plasmodium in excreta from groups of mosquitoes
    • Plasmodium in excreta, saliva and salivary glands 
    • Evaluation of parasite development in the mosquito - Parasite_dev_oocyst
    • Evaluation of parasite development in the mosquito - Parasite_dev_sporos
    • Survival analysis

    The full methodology is available in the Open Access publication from the Related Publications link below.

     

  • Other Descriptors
    • Descriptor

      This dataset is available as a spreadsheet saved in both MS Excel (.xlsx) and Open Document (.ods) formats.

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • mosquito
    • excreta
    • malaria
    • saliva
    • sporozoite
    • Plasmodium falciparum
    • Anopheles stephensi
  • Funding source
  • Research grant(s)/Scheme name(s)
  • Research themes
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 060808 - Invertebrate Biology
    • 060599 - Microbiology not elsewhere classified
    • 119999 - Medical and Health Sciences not elsewhere classified
    SEO Codes
    • 92 - Health
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date
  • End Date
  • Time Period
    Spatial (location) coverage
  • Locations
  • Related publications
      Name Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes
    • URL https://doi.org/10.1186/s13071-019-3610-9
    • Notes Open Access
  • Related websites
      Name
    • URL
    • Notes
  • Related metadata (including standards, codebooks, vocabularies, thesauri, ontologies)
  • Related data
      Name
    • URL
    • Notes
  • Related services
      Name
    • URL
    • Notes
    Citation Ramirez Lopez, Ana (2019): Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva. James Cook University. https://doi.org/10.25903/5d5de1e49aae9