Dataset describing detection or RRV and WNV in mosquito excreta

Mosquito excreta was collected from mosquitoes infected with arboviruses and tested for the presence of viral RNA by RT-rtPCR.

    Data Record Details
    Data record related to this publication Dataset describing detection or RRV and WNV in mosquito excreta
    Data Publication title Dataset describing detection or RRV and WNV in mosquito excreta
  • Description

    Mosquito excreta was collected from mosquitoes infected with arboviruses and tested for the presence of viral RNA by RT-rtPCR.

  • Other Descriptors
    • Descriptor

      Abstract [Related Publication]: Emerging and re-emerging arthropod-borne viruses (arboviruses) cause human and animal disease globally. Field and laboratory investigation of mosquito-borne arboviruses requires analysis of mosquito samples, either individually, in pools, or a body component, or secretion such as saliva. We assessed the applicability of mosquito excreta as a sample type that could be utilized during studies of Ross River and West Nile viruses, which could be applied to the study of other arboviruses. Mosquitoes were fed separate blood meals spiked with Ross River virus and West Nile virus. Excreta was collected daily by swabbing the bottom of containers containing batches and individual mosquitoes at different time points. The samples were analyzed by real-time RT-PCR or cell culture enzyme immunoassay. Viral RNA in excreta from batches of mosquitoes was detected continuously from day 2 to day 15 post feeding. Viral RNA was detected in excreta from at least one individual mosquito at all timepoints, with 64% and 27% of samples positive for RRV and WNV, respectively. Excretion of viral RNA was correlated with viral dissemination in the mosquito. The proportion of positive excreta samples was higher than the proportion of positive saliva samples, suggesting that excreta offers an attractive sample for analysis and could be used as an indicator of potential transmission. Importantly, only low levels of infectious virus were detected by cell culture, suggesting a relatively low risk to personnel handling mosquito excreta. Mosquito excreta is easily collected and provides a simple and efficient method for assessing viral dissemination, with applications ranging from vector competence experiments to complementing sugar-based arbovirus surveillance in the field, or potentially as a sample system for virus discovery.

      The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

      • Description (parameters)
      • RRV-groups
      • WNV-groups
      • RRV-individuals
      • WNV-individuals Viability of viral RNA in mosquito excreta

      The full methodology is available in the Open Access publication from the Related Publications link below.

    • Descriptor type Full
    • Descriptor

      This dataset is available as a spreadsheet saved in both MS Excel (.xlsx) and Open Document (.ods) formats.

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • mosquito
    • excreta
    • Ross River virus
    • West Nile virus
    • arbovirus
  • Funding source
  • Research grant(s)/Scheme name(s)
  • Research themes
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 069999 - Biological Sciences not elsewhere classified
    • 119999 - Medical and Health Sciences not elsewhere classified
    SEO Codes
    • 929999 - Health not elsewhere classified
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date
  • End Date
  • Time Period
    Spatial (location) coverage
  • Locations
  • Related publications
      Name Ramirez Lopez, Ana L., Hall-Mendelin, Sonja, Doggett, Stephen L., Hewitson, Glen R., Mcmahon, Jamie L., Ritchie, Scott A., and van den Hurk, Andrew F. (2018) Mosquito excreta: a sample type with many potential applications for the investigation of Ross River virus and West Nile virus ecology. PLoS Neglected Tropical Diseases, 12 (8). e0006771.
    • URL https://doi.org/10.1371/journal.pntd.0006771
    • Notes Open Access
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    • Notes
    Citation Ramirez Lopez, Ana (2019): Dataset describing detection or RRV and WNV in mosquito excreta. James Cook University. https://doi.org/10.25903/5d5f31376caa4