Tilapia eDNA (environmental DNA) data

Environmental DNA data from Robson HLA, Noble TH, Saunders RJ, Robson SKA, Burrows DW, Jerry DR (2016) Fine tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems.

Data consists of 4 files:

(1) Presence/absence data for filtration experiment 1 using 3, 10 and 20 um filters, with and without a prefiltering step

(2) Initial tilapia eDNA concentrations in ng at the start of Exp 2 on degradation for all three treatments, 23, 29 and 35 degrees Celcius

(3) Presence/absence data for Exp 2 on degradation of tilapia eDNA for 3 treatments of temperature 23,29 and 35 degrees Celcius

(4) Presence/absence data recorded as probability of detection for all qPCR replicates by day for all treatment pond densities (0,1,2,4,8,16,32, and up to 64 fish) for Experiment 3-Accumulation of tilapia eDNA.

Extract from Abstract [Related publication]: Invasive species pose a major threat to aquatic ecosystems. Their impact can be particularly severe in tropical regions, like those in northern Australia, where 20 invasive fish species are recorded. In temperate regions, environmental DNA (eDNA) technology is gaining momentum as a tool to detect aquatic pests, but the technology's effectiveness has not been fully explored in tropical systems with their unique climatic challenges (i.e. high turbidity, temperatures and ultraviolet light). In this study, we modified conventional eDNA protocols for use in tropical environments using the invasive fish, Mozambique tilapia (Oreochromis mossambicus) as a detection model. We evaluated the effects of high water temperatures and fish density on the detection of tilapia eDNA, using filters with larger pores to facilitate filtration.

The full methodology is available in the publication shown in the Related Publications link below.

    Data Record Details
    Data record related to this publication Tilapia eDNA (environmental DNA) data
    Data Publication title Tilapia eDNA (environmental DNA) data
  • Description

    Environmental DNA data from Robson HLA, Noble TH, Saunders RJ, Robson SKA, Burrows DW, Jerry DR (2016) Fine tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems.

    Data consists of 4 files:

    (1) Presence/absence data for filtration experiment 1 using 3, 10 and 20 um filters, with and without a prefiltering step

    (2) Initial tilapia eDNA concentrations in ng at the start of Exp 2 on degradation for all three treatments, 23, 29 and 35 degrees Celcius

    (3) Presence/absence data for Exp 2 on degradation of tilapia eDNA for 3 treatments of temperature 23,29 and 35 degrees Celcius

    (4) Presence/absence data recorded as probability of detection for all qPCR replicates by day for all treatment pond densities (0,1,2,4,8,16,32, and up to 64 fish) for Experiment 3-Accumulation of tilapia eDNA.

    Extract from Abstract [Related publication]: Invasive species pose a major threat to aquatic ecosystems. Their impact can be particularly severe in tropical regions, like those in northern Australia, where 20 invasive fish species are recorded. In temperate regions, environmental DNA (eDNA) technology is gaining momentum as a tool to detect aquatic pests, but the technology's effectiveness has not been fully explored in tropical systems with their unique climatic challenges (i.e. high turbidity, temperatures and ultraviolet light). In this study, we modified conventional eDNA protocols for use in tropical environments using the invasive fish, Mozambique tilapia (Oreochromis mossambicus) as a detection model. We evaluated the effects of high water temperatures and fish density on the detection of tilapia eDNA, using filters with larger pores to facilitate filtration.

    The full methodology is available in the publication shown in the Related Publications link below.

  • Other Descriptors
    • Descriptor

      This dataset is available from Dryad in MS Excel (.xls) format. Dryad data package: Robson HLA, Noble TH, Saunders RJ, Robson SKA, Burrows DW, Jerry DR (2016) Data from: Fine tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.6n5s3

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • environmental DNA
    • eDNA filtration
    • cichlid
    • Oreochromis mossambicus
    • Mozambique tilapia
    • invasive fish species
  • Funding source
  • Research grant(s)/Scheme name(s)
  • Research themes
    Tropical Ecosystems, Conservation and Climate Change
    FoR Codes (*)
    • 060204 - Freshwater Ecology
    • 070401 - Aquaculture
    SEO Codes
    • 960506 - Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date
  • End Date
  • Time Period
    Spatial (location) coverage
  • Locations
    Data Locations
    The Data Manager is: Simon Robson
    College or Centre
    Access conditions Open
  • Alternative access conditions
  • Data record size 4 files: 127.41 KB
  • Related publications
      Name Robson, Heather L.A., Noble, Tansyn H., Saunders, Richard J., Robson, Simon K.A., Burrows, Damien W., and Jerry, Dean R. (2016) Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems. Molecular Ecology, 16 (4). pp. 922-932.
    • URL http://dx.doi.org/10.1111/1755-0998.12505
    • Notes
  • Related websites
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    • URL
    • Notes
  • Related metadata (including standards, codebooks, vocabularies, thesauri, ontologies)
  • Related data
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    • Notes
    Citation Robson, Heather; Noble, Tansyn; Saunders, Richard; Robson, Simon; Burrows, Damien; Jerry, Dean (2016): Tilapia eDNA (environmental DNA) data. James Cook University.