Dataset describing stability of WNV RNA in mosquito excreta

 Abstract [Related Publication]: Arbovirus surveillance is crucial for the implementation of vector-borne disease control measures. Recently, it has been demonstrated that mosquitoes with a disseminated arbovirus infection excrete viral RNA, which can be detected by molecular methods. Thereby, mosquito excreta has been proposed as a sample type that could be utilized for arbovirus surveillance. In this study, we evaluated if West Nile virus (Kunjin strain, WNVKUN) RNA in Culex annulirostris Skuse (Diptera: Culicidae) excreta deposited on different substrates could be detected after storage for up to 2 wk at tropical conditions of high heat and humidity. No significant drop in relative quantity of WNVKUN RNA (determined by comparison of Ct values) in excreta deposited on Flinders Associate Technologies (FTA) cards was observed over 14 d, suggesting that RNA was stable for that time. There was no significant difference in relative quantity of WNVKUN RNA in excreta deposited on FTA cards or polycarbonate substrates after 24 h. However, after 7 and 14 d, there was a significant decline in the relative quantity of viral RNA in the excreta stored on polycarbonate substrates. For incorporation in arbovirus surveillance programs, we recommend the use of polycarbonate substrates for excreta collection in mosquito traps deployed overnight, and the integration of FTA cards in traps serviced weekly or fortnightly. Polycarbonate substrates facilitate the collection of the majority of excreta from a trap, and while FTA cards offer limited area coverage, they enable preservation of viral RNA in tropical conditions for extended periods of time.

The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

  • RRV-groups
  • WNV-groups
  • RRV-individuals
  • WNV-individuals
  • Viability of viral RNA in mosquito excreta

 The full methodology is available in the publication shown in the Related Publications link below.

    Data Record Details
    Data record related to this publication Dataset describing stability of WNV RNA in mosquito excreta
    Data Publication title Dataset describing stability of WNV RNA in mosquito excreta
  • Description

     Abstract [Related Publication]: Arbovirus surveillance is crucial for the implementation of vector-borne disease control measures. Recently, it has been demonstrated that mosquitoes with a disseminated arbovirus infection excrete viral RNA, which can be detected by molecular methods. Thereby, mosquito excreta has been proposed as a sample type that could be utilized for arbovirus surveillance. In this study, we evaluated if West Nile virus (Kunjin strain, WNVKUN) RNA in Culex annulirostris Skuse (Diptera: Culicidae) excreta deposited on different substrates could be detected after storage for up to 2 wk at tropical conditions of high heat and humidity. No significant drop in relative quantity of WNVKUN RNA (determined by comparison of Ct values) in excreta deposited on Flinders Associate Technologies (FTA) cards was observed over 14 d, suggesting that RNA was stable for that time. There was no significant difference in relative quantity of WNVKUN RNA in excreta deposited on FTA cards or polycarbonate substrates after 24 h. However, after 7 and 14 d, there was a significant decline in the relative quantity of viral RNA in the excreta stored on polycarbonate substrates. For incorporation in arbovirus surveillance programs, we recommend the use of polycarbonate substrates for excreta collection in mosquito traps deployed overnight, and the integration of FTA cards in traps serviced weekly or fortnightly. Polycarbonate substrates facilitate the collection of the majority of excreta from a trap, and while FTA cards offer limited area coverage, they enable preservation of viral RNA in tropical conditions for extended periods of time.

    The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

    • RRV-groups
    • WNV-groups
    • RRV-individuals
    • WNV-individuals
    • Viability of viral RNA in mosquito excreta

     The full methodology is available in the publication shown in the Related Publications link below.

  • Other Descriptors
    • Descriptor

      This dataset is available as a spreadsheet saved in both MS Excel (.xlsx) and Open Document (.ods) formats.

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • mosquito
    • arbovirus
    • excreta
    • RNA stability
    • surveillance
  • Funding source
  • Research grant(s)/Scheme name(s)
  • Research themes
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 069999 - Biological Sciences not elsewhere classified
    • 119999 - Medical and Health Sciences not elsewhere classified
    SEO Codes
    • 929999 - Health not elsewhere classified
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date
  • End Date
  • Time Period
    Spatial (location) coverage
  • Locations
  • Related publications
      Name Ramírez, Ana L., Hall-Mendelin, Sonja, Hewitson, Glen R., McMahon, Jamie L., Staunton, Kyran M., Ritchie, Scott A., and van den Hurk, Andrew F. (2019) Stability of West Nile Virus (Flaviviridae: Flavivirus) RNA in mosquito excreta. Journal of Medical Entomology, 56 (4). pp. 1135-1138.
    • URL https://doi.org/10.1093/jme/tjz044
    • Notes
  • Related websites
      Name
    • URL
    • Notes
  • Related metadata (including standards, codebooks, vocabularies, thesauri, ontologies)
  • Related data
      Name
    • URL
    • Notes
  • Related services
      Name
    • URL
    • Notes
    Citation Ramirez Lopez, Ana (2019): Dataset describing stability of WNV RNA in mosquito excreta. James Cook University. https://doi.org/10.25903/5d5f2a38e823f