In vitro percutaneous absorption data for model chemicals in Litoria caerulea and Rhinella marina: chemicals formulated in Amphibian Ringer’s solution

Percutaneous absorption kinetics of caffeine, benzoic acid, and ibuprofen through isolated skin from the green tree frog Litoria caerulea, and the terrestrial toad Rhinella marina.

 

    Data Record Details
    Data record related to this publication In vitro percutaneous absorption data for model chemicals in Litoria caerulea and Rhinella marina: chemicals formulated in Amphibian Ringer’s solution
    Data Publication title In vitro percutaneous absorption data for model chemicals in Litoria caerulea and Rhinella marina: chemicals formulated in Amphibian Ringer’s solution
  • Description

    Percutaneous absorption kinetics of caffeine, benzoic acid, and ibuprofen through isolated skin from the green tree frog Litoria caerulea, and the terrestrial toad Rhinella marina.

     

  • Other Descriptors
    • Descriptor

      Although it is accepted that frogs have highly-permeable skin, little is known how the physicochemical characteristics of chemicals, or skin region of chemical application, influence percutaneous absorption in frogs. Additionally, frog species from different primary habitats have different skin composition, thickness and vascularisation, and this is also likely to influence percutaneous absorption. This dataset provides in vitro absorption kinetics for three model chemicals - benzoic acid, caffeine, and ibuprofen - through excised skin of two frog species: the arboreal frog Litoria caerulea and the terrestrial toad Rhinella marina. Franz-type diffusion cells were used to measure absorption, and chemical content was quantified using validated HPLC methods.

      Each chemical was formulated as a saturated solution (infinite dose) in Amphibian Ringer’s solution and applied to the external skin surface. Diffusion through the skin was measured over a period of six hours for benzoic acid and caffeine, and for 24 hours for ibuprofen. Chemical flux was determined from the steady-state slope of the cumulative absorption vs time plot.

      Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, species, sex, weight, and from which body regions each skin sample was obtained), chemical information including name, concentration, logP and molecular weight, and absorption data including chemical content at each sample time and calculated flux and Kp values.

      Column headings are explained below:

      AnimalID: individual animal identifier

      Conc: concentration of chemical applied (microgram/ml)

      Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen)

      LogP: logarithm of the partition coefficient for chemical investigated

      MW: molecular weight of the chemical investigated

      Species: frogs species used (L. caerulea or Rh. marina)

      Sex: frog sex

      Weight: frog weight in grams

      Region: primary skin region (dorsal=D/ ventral=V) of skin sample

      SubRegion: For Rh. marina, identifies if ventral samples are thoracic (T) or pelvic (P)  in origin. Otherwise same as Region (Dorsal=D, Ventral=V)

      LeftRightMid: identifies if samples taken from left (L), right (R) or on the midline (M)

      Flux: steady-state flux of the chemical

      logFlux: logarithm of flux

      Kp: permeability coefficient of the chemical (Kp=Conc/Flux)

      t=0 through t=24: concentration (microgram/ml) of chemical in receptor fluid at each sampling time (hr)

    • Descriptor type Full
  • Data type dataset
  • Keywords
    • Anura
    • skin absorption
    • amphibian
    • toxicokinetics
    • wildlife toxicology
    • octanol–water partition coefficient
    • Anura
  • Funding source
  • Research grant(s)/Scheme name(s)
  • Research themes
    Tropical Ecosystems, Conservation and Climate Change
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 070710 - Veterinary Pharmacology
    • 111504 - Pharmaceutical Sciences
    SEO Codes
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date 2016/12/10
  • End Date 2017/04/07
  • Time Period
    Spatial (location) coverage
  • Locations
    Data Locations

    Type Location Notes
    Attachment INVITRO_LCRMl.csv
    The Data Manager is:
    College or Centre
    Access conditions Open
  • Alternative access conditions
  • Data record size 1 file: 24 KB
  • Related publications
      Name Llewelyn, Victoria K., Berger, Lee, and Glass, Beverley D. (2019) Effects of skin region and relative lipophilicity on percutaneous absorption in the toad Rhinella marina. Environmental Toxicology and Chemistry, 38 (2). pp. 361-367.
    • URL https://doi.org/10.1002/etc.4302
    • Notes
    • Name Llewelyn, Victoria K., Berger, Lee, and Glass, Beverley D. (2018) Regional variation in percutaneous absorption in the tree frog Litoria caerulea. Environmental Toxicology and Pharmacology, 60. pp. 5-11.
    • URL https://doi.org/10.1016/j.etap.2018.03.019
    • Notes
  • Related websites
      Name
    • URL
    • Notes
  • Related metadata (including standards, codebooks, vocabularies, thesauri, ontologies)
  • Related data
      Name
    • URL
    • Notes
  • Related services
      Name
    • URL
    • Notes
    Citation Llewelyn, Victoria (2019): In vitro percutaneous absorption data for model chemicals in Litoria caerulea and Rhinella marina: chemicals formulated in Amphibian Ringer’s solution. James Cook University. https://doi.org/10.25903/5d4a64751b5de