In vitro percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in penetration enhancers ethanol or propylene glycol

Previous studies by the authors measured in vitro absorption kinetics of model chemicals through Rh. marina skin when the chemicals were formulated as saturated solutions in Amphibian Ringers Solution. However, many chemicals are formulated in solutions containing penetration enhancers. No data exists to quantify the effect of penetration enhancers on absorption kinetics through frog skin, nor is there information of how these enhancers effect frog skin structure. This dataset provides in vitro absorption kinetics for three model chemicals - benzoic acid, caffeine, and ibuprofen – formulated in either 1/10/30% v/v ethanol or 20% PG solution. Franz-type diffusion cells were used to measure absorption, and chemical content was quantified using validated HPLC methods. Additionally, the impact on frog skin due to exposure to these penetration enhancers was characterised using differential scanning calorimetry (DSC) and histology. 

Each chemical was formulated as a saturated solution (infinite dose) in one of the enhancers and applied to the external skin surface. Diffusion through the skin was measured over a period of four to six hours. Chemical flux was determined from the steady-state slope of the cumulative absorption vs time plot. Skin samples for DSC experiments and histology were either freshly excised, or exposed to one of the following for a period of six hours: ARS, 1/10/30% v/v ethanol, 20% v/v PG.

Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, sex, weight, and from which body regions each skin sample was obtained), penetration enhancer identification, chemical information (including name and concentration) and absorption data including chemical content at each sample time and calculated flux and Kp values. DSC and histology are available by contacting the author.

Column headings for the spreadsheet are explained below:

AnimalID: individual animal identifier

Sex: animal sex 

Weight: frog weight in grams

Region: primary skin region (dorsal=D/ ventral=V) of skin sample

SubRegion: Identifies if ventral samples are thoracic (T) or pelvic (P) in origin. Dorsal samples remain coded as “D”

LeftRightMid: identifies if samples taken from left (L), right (R) or on the midline (M)

Solvent: enhancer used (1/10/30% v/v ethanol or 20% PG)

Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen)

Conc: concentration of chemical applied (microgram/ml)

Flux: steady-state flux of the chemical

Kp: permeability coefficient of the chemical (Kp=Conc/Flux)

t=0 through t=6: concentration (microgram/ml) of chemical in receptor fluid at each sampling time (hr)

 

    Data Record Details
    Data record related to this publication In vitro percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in penetration enhancers ethanol or propylene glycol
    Data Publication title In vitro percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in penetration enhancers ethanol or propylene glycol
  • Description

    Previous studies by the authors measured in vitro absorption kinetics of model chemicals through Rh. marina skin when the chemicals were formulated as saturated solutions in Amphibian Ringers Solution. However, many chemicals are formulated in solutions containing penetration enhancers. No data exists to quantify the effect of penetration enhancers on absorption kinetics through frog skin, nor is there information of how these enhancers effect frog skin structure. This dataset provides in vitro absorption kinetics for three model chemicals - benzoic acid, caffeine, and ibuprofen – formulated in either 1/10/30% v/v ethanol or 20% PG solution. Franz-type diffusion cells were used to measure absorption, and chemical content was quantified using validated HPLC methods. Additionally, the impact on frog skin due to exposure to these penetration enhancers was characterised using differential scanning calorimetry (DSC) and histology. 

    Each chemical was formulated as a saturated solution (infinite dose) in one of the enhancers and applied to the external skin surface. Diffusion through the skin was measured over a period of four to six hours. Chemical flux was determined from the steady-state slope of the cumulative absorption vs time plot. Skin samples for DSC experiments and histology were either freshly excised, or exposed to one of the following for a period of six hours: ARS, 1/10/30% v/v ethanol, 20% v/v PG.

    Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, sex, weight, and from which body regions each skin sample was obtained), penetration enhancer identification, chemical information (including name and concentration) and absorption data including chemical content at each sample time and calculated flux and Kp values. DSC and histology are available by contacting the author.

    Column headings for the spreadsheet are explained below:

    AnimalID: individual animal identifier

    Sex: animal sex 

    Weight: frog weight in grams

    Region: primary skin region (dorsal=D/ ventral=V) of skin sample

    SubRegion: Identifies if ventral samples are thoracic (T) or pelvic (P) in origin. Dorsal samples remain coded as “D”

    LeftRightMid: identifies if samples taken from left (L), right (R) or on the midline (M)

    Solvent: enhancer used (1/10/30% v/v ethanol or 20% PG)

    Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen)

    Conc: concentration of chemical applied (microgram/ml)

    Flux: steady-state flux of the chemical

    Kp: permeability coefficient of the chemical (Kp=Conc/Flux)

    t=0 through t=6: concentration (microgram/ml) of chemical in receptor fluid at each sampling time (hr)

     

  • Other Descriptors
    • Descriptor

      Effect of penetration enhancers on the percutaneous absorption kinetics of caffeine, benzoic acid, and ibuprofen through isolated skin from the terrestrial toad Rhinella marina.

    • Descriptor type Brief
  • Data type dataset
  • Keywords
    • skin absorption
    • Anuran
    • amphibian
    • propylene glycol
    • ethanol
    • Differential Scanning Calorimetry
    • histology
    • in vitro techniques
  • Funding source
  • Research grant(s)/Scheme name(s)
  • Research themes
    Tropical Ecosystems, Conservation and Climate Change
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 070710 - Veterinary Pharmacology
    • 111504 - Pharmaceutical Sciences
    SEO Codes
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    Temporal (time) coverage
  • Start Date 2017/02/21
  • End Date 2018/09/13
  • Time Period
    Spatial (location) coverage
  • Locations
    Data Locations

    Type Location Notes
    Physical Location Research Data JCU secure CloudStor account
    The Data Manager is:
    College or Centre
    Access conditions Conditional
  • Alternative access conditions
  • Data record size 1 file: 21 KB
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  • Other Licence
  • Statement of rights in data Once access to the data has been obtained via negotiation with the data manager, use of the dataset is governed by the CC-BY-NC licence.
  • Data owners
      Victoria Llewelyn
    Citation Llewelyn, Victoria (2019): In vitro percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in penetration enhancers ethanol or propylene glycol. James Cook University. https://doi.org/10.25903/5d4a6ff6bae14