Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas)

These datasets contain proteins identified by mass spectrometry in the raw and the heated sample of Pacific oyster (C. gigas)

    Data Record Details
    Data record related to this publication Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas)
    Data Publication title Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas)
  • Description

    These datasets contain proteins identified by mass spectrometry in the raw and the heated sample of Pacific oyster (C. gigas)

  • Other Descriptors
    • Descriptor

      Protein compositions of the Pacific oyster extracts were identified through bottom-up mass spectrometry approach. The proteins were separated on an SDS-PAGE gel, cut into small pieces and tryptic digested (Fig 3A). The peptide mixtures were then subjected to LC-MS/MS analysis generating a total of 81831 spectra for the raw extract and 88130 spectra for the heated extract. Mascot searches were conducted against Pacific oyster proteomes, and the identified proteins were validated by Scaffold (version Scaffold_4.7.3) using criteria at least two peptides at a 99.0% probability. In total Scaffold identified 1,086 proteins from raw extract and 130 proteins from the heated extract, with 103 proteins are shared between the two extracts.

    • Descriptor type Full
    • Descriptor

      This dataset consists of a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • Pacific oyster
    • proteome
    • mass spectrometry
  • Funding source
  • Research grant(s)/Scheme name(s)
    • FT110100635 - ARC - Molecular and immunological approaches to managing Australia’s seafood allergy epidemic
  • Research themes
    Industries and Economies in the Tropics
    People and Societies in the Tropics
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 110701 - Allergy
    • 110106 - Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
    SEO Codes
    • 920108 - Immune System and Allergy
    • 920203 - Diagnostic Methods
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date 2017/08/23
  • End Date
  • Time Period
    Spatial (location) coverage
  • Locations
    Data Locations

    Type Location Notes
    Physical Location Secure data section of the Tropical Data Hub (TDH) archive - eResearch Centre, James Cook University, Townsville, Queensland, Australia
    The Data Manager is: Andreas Lopata
    College or Centre
    Access conditions Conditional: Contact researchdata@jcu.edu.au to request access to this data.
  • Alternative access conditions
  • Data record size 486.75 KB
  • Related publications
      Name Nugreaha, Roni, Kamath, Sandip D., Ruethers, Thimo, Johnston, Elecia, Koeberl, Martina, Rolland, Jennifer M., O'Hehir, Robyn E., Zenger, Kyall R., and Lopata, Andreas L. (2016). Identification of novel oyster allergens using a combined transcriptomic and proteomic approach for improved component resolved diagnosis. Internal Medicine Journal, 46 (Suppl. 4). pp. 9.
    • URL http://dx.doi.org/10.1111/imj.16_13197
    • Notes ASCIA-2016 Published Abstract
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    Select or add a licence for the data
    The data will be licensed under CC BY-NC: Attribution-Noncommercial 3.0 AU
  • Other Licence
  • Statement of rights in data Once access to the data has been obtained via negotiation with the data manager, use of the dataset is governed by the CC-BY-NC AU licence.
  • Data owners
      James Cook University
    Citation Lopata, Andreas; Kamath, Sandip; Nugraha, Roni (2017): Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas). James Cook University. https://doi.org/10.4225/28/588eb674aa910