The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome

Data from the National Center for Biolotechnology Information, Gene Expression Omnibus GEO accession: GSE76976

Organism: Acropora digitifera
Experiment type: Expression profiling by high throughput sequencing
Summary: Despite the ecological significance of the relationship between reef-building corals and intracellular photosynthetic dinoflagellates of the genus Symbiodinium, very little is known about the molecular mechanisms involved in the establishment of the relationship. Indeed, microarray-based analyses point to the conclusion that host gene expression is largely or completely unresponsive during the establishment of symbiosis with a competent strain of Symbiodinium. In the present study, the use of Illumina RNAseq technology allowed detection of a transient period of differential expression involving a small number of genes (1073 transcripts; <3% of the transcriptome) 4h after the exposure of Acropora digitifera planulae to a competent strain of Symbiodinium (a clade B strain). This phenomenon has not previously been detected as a consequence of both the lower sensitivity of the microarray approaches used and the sampling times used. The results imply that complex changes occur, including transient suppression of mitochondrial metabolism and protein synthesis, but are also consistent with the hypothesis that the symbiosome is a phagosome that has undergone early arrest, raising the possibility of common mechanisms in the symbiotic interactions of corals and symbiotic sea anemones with their endosymbionts.
     
Overall design: There were 2 conditions (Symbiodinium-infected and control). Samples were taken at 3 time points, there were 3 replicates per condition. 16 samples were analysed comparing the Symbiodinium-infected samples to the control ones.

    Data Record Details
    Data record related to this publication The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome
    Data Publication title The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome
  • Description

    Data from the National Center for Biolotechnology Information, Gene Expression Omnibus GEO accession: GSE76976

    Organism: Acropora digitifera
    Experiment type: Expression profiling by high throughput sequencing
    Summary: Despite the ecological significance of the relationship between reef-building corals and intracellular photosynthetic dinoflagellates of the genus Symbiodinium, very little is known about the molecular mechanisms involved in the establishment of the relationship. Indeed, microarray-based analyses point to the conclusion that host gene expression is largely or completely unresponsive during the establishment of symbiosis with a competent strain of Symbiodinium. In the present study, the use of Illumina RNAseq technology allowed detection of a transient period of differential expression involving a small number of genes (1073 transcripts; <3% of the transcriptome) 4h after the exposure of Acropora digitifera planulae to a competent strain of Symbiodinium (a clade B strain). This phenomenon has not previously been detected as a consequence of both the lower sensitivity of the microarray approaches used and the sampling times used. The results imply that complex changes occur, including transient suppression of mitochondrial metabolism and protein synthesis, but are also consistent with the hypothesis that the symbiosome is a phagosome that has undergone early arrest, raising the possibility of common mechanisms in the symbiotic interactions of corals and symbiotic sea anemones with their endosymbionts.
         
    Overall design: There were 2 conditions (Symbiodinium-infected and control). Samples were taken at 3 time points, there were 3 replicates per condition. 16 samples were analysed comparing the Symbiodinium-infected samples to the control ones.

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  • Data type dataset
  • Keywords
    • Acropora
    • Symbiodinium
    • symbiosome
    • transcriptomics
    • RNA-Seq
    • transcriptome
    • gene expression
    • coral-algal symbiosis
    • ARC Centre of Excellence for Coral Reef Studies
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  • Research themes
    Tropical Ecosystems, Conservation and Climate Change
    FoR Codes (*)
    • 060405 - Gene Expression (incl. Microarray and other genome-wide approaches)
    • 060205 - Marine and Estuarine Ecology (incl. Marine Ichthyology)
    SEO Codes
    • 970106 - Expanding Knowledge in the Biological Sciences
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      Name Mohamed, A.R., Cumbo, V., Harii, S., Shinzato, C., Chan, C.X., Ragan, M.A., Bourne, D.G., Willis, B.L., Ball, E.E., Satoh, N., and Miller, D.J. (2016) The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome. Molecular Ecology, 25 (13). pp. 3127-3141.
    • URL http://dx.doi.org/10.1111/mec.13659
    • Notes
    • Name Chapter 2 of PhD thesis: Transcriptomics of coral-algal interactions: novel insights into the establishment of symbiosis
    • URL https://researchonline.jcu.edu.au/44212/
    • Notes Accepted Version of Molecular Ecology paper included in PhD thesis; Open Access
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      James Cook University
    Citation Mohamed Esmail, Amin; Cumbo, Vivian; Willis, Bette; Ball, Eldon; Miller, David (2016): The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome. James Cook University.