Potential allergens of the Pacific Oyster (Crassostrea gigas)

The increasing production and consumption of molluscs are associated with a rise in the prevalence of mollusc allergy worldwide, currently affecting 0.2% to 1.3% of the general population. However, the elucidation of mollusc allergens, crucial for better diagnostics, still lags behind other seafood groups such as fish and crustacean. Genomic data have previously been utilized for the improved identification of non-food allergens by performing similarity searches using the BLAST program. Based on the published genome of the Pacific oyster (Crassostrea gigas) we aimed to identify the repertoire of potential allergen using bioinformatics analysis and sought to validate allergenicity using a combination of immuno-chemical methods and proteomic analysis. A repertoire of 25,982 genome-derived proteomes of the Pacific oyster were aligned with 2117 allergen sequences resulting in over 800 protein homologues. Of those, 95 proteins were potentially cross-reactive allergens due to high identity with known allergens (>50% identity). Analysis of the transcriptomic data showed the proteins were differentially expressed across tissue of the oyster.

    Data Record Details
    Data record related to this publication Potential allergens of the Pacific Oyster (Crassostrea gigas)
    Data Publication title Potential allergens of the Pacific Oyster (Crassostrea gigas)
  • Description

    The increasing production and consumption of molluscs are associated with a rise in the prevalence of mollusc allergy worldwide, currently affecting 0.2% to 1.3% of the general population. However, the elucidation of mollusc allergens, crucial for better diagnostics, still lags behind other seafood groups such as fish and crustacean. Genomic data have previously been utilized for the improved identification of non-food allergens by performing similarity searches using the BLAST program. Based on the published genome of the Pacific oyster (Crassostrea gigas) we aimed to identify the repertoire of potential allergen using bioinformatics analysis and sought to validate allergenicity using a combination of immuno-chemical methods and proteomic analysis. A repertoire of 25,982 genome-derived proteomes of the Pacific oyster were aligned with 2117 allergen sequences resulting in over 800 protein homologues. Of those, 95 proteins were potentially cross-reactive allergens due to high identity with known allergens (>50% identity). Analysis of the transcriptomic data showed the proteins were differentially expressed across tissue of the oyster.

  • Other Descriptors
    • Descriptor

      This dataset consists of a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • food allergens
    • oyster
    • genomic
    • in-silico
    • immunoinformatics
  • Funding source
  • Research grant(s)/Scheme name(s)
    • FT110100635 - ARC - Molecular and immunological approaches to managing Australia’s seafood allergy epidemic
  • Research themes
    Industries and Economies in the Tropics
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    • 110701 - Allergy
    • 110106 - Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
    SEO Codes
    • 920108 - Immune System and Allergy
    • 920203 - Diagnostic Methods
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    Temporal (time) coverage
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  • Locations
    Data Locations

    Type Location Notes
    Physical Location Secure data section of the Tropical Data Hub (TDH) archive - eResearch Centre, James Cook University, Townsville, Queensland, Australia
    The Data Manager is: Andreas Lopata
    College or Centre
    Access conditions Conditional: Contact researchdata@jcu.edu.au to request access to this data.
  • Alternative access conditions
  • Data record size 1.6 MB
  • Related publications
      Name Nugraha, Roni, Zenger, Kyall, Kamath, Sandip D. and Lopota, Andreas L. (2015). In-silico bioinformatics approach for the identification and characterisation of allergens from the Pacific oyster (Crassotrea gigas). Internal Medicine Journal, 45 (Suppl. 4). pp. 9.
    • URL http://dx.doi.org/10.1111/imj.12869
    • Notes ASCIA-2015 Published Abstract
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    Select or add a licence for the data
    The data will be licensed under CC BY-NC: Attribution-Noncommercial 3.0 AU
  • Other Licence
  • Statement of rights in data Once access to the data has been obtained via negotiation with the data manager, use of the dataset is governed by the CC-BY-NC AU licence.
  • Data owners
      James Cook University
    Citation Lopata, Andreas; Kamath, Sandip; Nugraha, Roni (2017): Potential allergens of the Pacific Oyster (Crassostrea gigas). James Cook University. https://doi.org/10.4225/28/588ebc2ca00e2