JCU > Research Portfolio > Dr Javier Sotillo-Gallego

About

I obtained my PhD in 2011 at University of Valencia (Spain) with high honours under the supervision of Dr. Rafael Toledo and J. Guillermo Esteban. My interests during my PhD were the host-parasite interactions at a proteomics and immunological level in the Echinostoma-rodent. In 2012 I was appointed as a Postdoctoral Research Fellow at James Cook University at the laboratory of Alex Loukas.

My research focus is the analysis of the proteomes and secretomes of parasites and how they orchestrate a parasitic existence. I am also interested in the host-parasite relationships, with a particular emphasis on soil-transmitted helminths (e.g. hookworms, whipworms, roundworms) and water-borne trematodes such as Schistosoma spp. I have described the excretory-secretory proteins of different helminths and have been involved in in analyses of large-scale sequence datasets generated from a range of parasites (i.e. transcriptomes). I recently focused my attention to study small-secreted parasitic vesicles (exosomes) and their implication in parasite-host communication and the modulation of the host’s immune response.

Interests

Research

Proteomics of helminths
Host-parasite interactions

Experience

2013 to present - Postdoctoral Research Fellow, James Cook University (Cairns, Australia)
2012 to 2013 - Proteomics Laboratory Manager, James Cook University (Cairns, Australia)
2006 to 2010 - PhD student, University of Valencia (Valencia, Spain)
2008 - Visiting scientist, The Queen's University of Belfast (Belfast, Northern Ireland)

Research Disciplines

Socio-Economic Objectives

Honours

Fellowships

2006 to 2010 - PhD fellowship by Ministry of Education (Spain)
2004 to 2005 - Honours student fellowship

Memberships

2013 - Current member of the Australian Society for Parasitology
2008 - Current member of the Spanish Society for Proteomics
2006 - Curent member of the Spanish Society for Parasitology

Other

2013 - Reviewer for scientific journals such as PLoS NTD and Journal of Helminthology
2011 to 2012 - Grant reviewer for the Spanish Agency of Evaluation and Research (ANEP)

Publications

These are the most recent publications associated with this author. To see a detailed profile of all publications stored at JCU, visit ResearchOnline@JCU. Hover over Altmetrics badges to see social impact.

Journal Articles

Khudhair Z, Alhallaf R, Eichenberger R, Whan J, Kupz A, Field M, Krause L, Wilson D, Daly N, Giacomin P, Sotillo J and Loukas A (2021) Gastrointestinal helminth infection improves insulin sensitivity, decreases systemic inflammation, and alters the composition of gut microbiota in distinct mouse models of Type 2 diabetes. Frontiers in Endocrinology, 11, Article: 606530, DOI:10.3389/fendo.2020.606530.
Arunsan P, Chaidee A, Cochran C, Mann V, Tanno T, Kumkhaek C, Smout M, Karinshak S, Rodpai R, Sotillo J, Loukas A, Laha T, Brindley P and Ittiprasert W (2020) Liver fluke granulin promote extracellular vesicle-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma. Neoplasia, 22 (5), pp. 203-216, DOI:10.1016/j.neo.2020.02.004.
Bouchery T, Moyat M, Sotillo J, Silverstein S, Volpe B, Coakley G, Tsourouktsoglou T, Becker L, Shah K, Kulagin M, Guiet R, Camberis M, Schmidt A, Seitz A, Giacomin P, Le Gros G, Papayannopoulos V, Loukas A and Harris N (2020) Hookworms evade host immunity by secreting a deoxyribonuclease to degrade neutrophil extracellular traps. Cell Host & Microbe, 27 (2), pp. 277-289.e6, DOI:10.1016/j.chom.2020.01.011.
González-Miguel J, Becerro-Recio D, Sotillo J, Simón F and Siles-Lucas M (2020) Set up of an in vitro model to study early host-parasite interactions between newly excysted juveniles of Fasciola hepatica and host intestinal cells using a quantitative proteomics approach. Veterinary Parasitology, 278, Article: 109028, DOI:10.1016/j.vetpar.2020.109028.
Kifle D, Pearson M, Becker L, Pickering D, Loukas A and Sotillo J (2020) Proteomic analysis of two populations of Schistosoma mansoni-derived extracellular vesicles: 15k pellet and 120k pellet vesicles. Molecular and Biochemical Parasitology, 236, Article: 111264, DOI:10.1016/j.molbiopara.2020.111264.
Logan J, Pearson M, Manda S, Choi Y, Field M, Eichenberger R, Mulvenna J, Nagaraj S, Fujiwara R, Gazzinelli-Guimaraes P, Bueno L, Mati V, Bethony J, Mitreva M, Sotillo J and Loukas A (2020) Comprehensive analysis of the secreted proteome of adult Necator americanus hookworms. PLoS Neglected Tropical Diseases, 14 (5), Article: e0008237, DOI:10.1371/journal.pntd.0008237.
Mekonnen G, Tedla B, Pickering D, Becker L, Wang L, Zhan B, Bottazzi M, Loukas A, Sotillo J and Pearson M (2020) Schistosoma haematobium extracellular vesicle proteins confer protection in a heterologous model of schistosomiasis. Vaccines, 8 (3), Article: 416, DOI:10.3390/vaccines8030416.
Suwannatrai K, Suwannatrai A, Loukas A and Sotillo J (2020) Recent advances on the immunobiology of Bithynia spp. hosts of Opisthorchis viverrini. Developmental and Comparative Immunology, 102, Article: 103460, DOI:10.1016/j.dci.2019.103460.
Thi Phung L, Chaiyadet S, Hongsrichan N, Sotillo J, Dinh Thi Dieu H, Quang Tran C, Brindley P, Loukas A and Laha T (2020) Partial protection with a chimeric tetraspanin-leucine aminopeptidase subunit vaccine against Opisthorchis viverrini infection in hamsters. Acta Tropica, 204, Article: 105355, DOI:10.1016/j.actatropica.2020.105355.
Arunsan P, Ittiprasert W, Smout M, Cochran C, Mann V, Chaiyadet S, Karinshak S, Sripa B, Young N, Sotillo J, Loukas A, Brindley P and Laha T (2019) Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity. eLife, 8, Article: e41463, DOI:10.7554/eLife.41463.
Chaiyadet S, Sotillo J, Krueajampa W, Thongsen S, Brindley P, Sripa B, Loukas A and Laha T (2019) Vaccination of hamsters with Opisthorchis viverrini extracellular vesicles and vesicle-derived recombinant tetraspanins induces antibodies that block vesicle uptake by cholangiocytes and reduce parasite burden after challenge infection. PLoS Neglected Tropical Diseases, 13 (5), Article: e0007450, DOI:10.1371/journal.pntd.0007450.
Phung L, Chaiyadet S, Hongsrichan N, Sotillo J, Dieu H, Tran C, Brindley P, Loukas A and Laha T (2019) Recombinant Opisthorchis viverrini tetraspanin expressed in Pichia pastoris as a potential vaccine candidate for opisthorchiasis. Parasitology Research, 118, pp. 3419-3427, DOI:10.1007/s00436-019-06488-3.

More: ResearchOnline@JCU stores 71+ research outputs authored by Dr Javier Sotillo-Gallego from 2008 onwards.

Current Funding

Current and recent Research Funding to JCU is shown by funding source and project.

National Institute of Health - RO1

Carcinogenic liver fluke infection: Gene editing- and vaccination-mediated approaches to interrupt host-parasite communication

Indicative Funding: $825,674 over 5 years (administered by George Washington University)
Summary: Long term infection with liver fluke - a food-borne parasitic worm - leads to cholangiocarcinoma (CCA), a form of liver cancer with a dismal prognosis. Previously we identified proteins and vesicles from these parasites that may cause this cancer. This new project will investigate the roles of these parasite proteins and vesicles in cancer, which may lead to new treatments and control for fluke infection and CCA.
Investigators: Alex Loukas, Michael Smout and Javier Sotillo-Gallego (Australian Institute of Tropical Health & Medicine)
Keywords: Cancer; Infectious disease; Parasite; CRISPR/Cas9; Vaccines

National Health & Medical Research Council - Project Grant

Secreted exosome-like vesicles from the carcinogenic liver fluke

Indicative Funding: $742,660 over 4 years
Summary: Parasitic worms secrete molecules from their oral openings and outer surfaces as they feed and reproduce inside their human hosts. These molecules are referred to as Excretory/Secretory (ES) products, akin to our saliva and sweat. These ES products represent the molecular interface of the host-parasite relationship. We recently showed that ES products from the parasitic liver fluke, a worm that is a major cause of liver cancer throughout parts of SE Asia, are taken up by cells lining the human bile ducts, the site where the parasite resides for years at a time. Until now the mechanisms by which these molecules are taken up and internalised by host cells was unknown. We now show that liver fluke ES proteins enter into human bile duct cells by forming small cell-like vesicles called exosomes. Once the flukes exosomes get inside human bile duct cells they induce a series of changes inside the cell which typifies the early stages of cancer formation. We now propose to better characterise the process of exosome uptake by human bile duct cells and exploit this information to discover vaccines to combat this carcinogenic infection and develop new tools to identify people who are most at risk of developing cancer from liver fluke infection.
Investigators: Alex Loukas, Javier Sotillo-Gallego and Thewarch Laha in collaboration with Paul Brindley, Jeffrey Bethony, Banchob Sripa and Jason Mulvenna (College of Public Health, Medical & Vet Sciences, Australian Institute of Tropical Health & Medicine, Khon Kaen University, George Washington University and Queensland Institute of Medical Research)
Keywords: Opisthorchis viverrini; Liver Cancer; Exosome; Excretory/secretory; Bile duct

Supervision

Advisory Accreditation: I can be on your Advisory Panel as a Primary or Secondary Advisor.

These Higher Degree Research projects are either current or by students who have completed their studies within the past 5 years at JCU. Linked titles show theses available within ResearchOnline@JCU.

Completed

Impact of hookworms and their secreted proteins on the microbiota and subsequent development of type 2 diabetes in mice (2020, PhD , Secondary Advisor)
Characterisation of the proteomic composition of Schistosoma haematobium extracellular vesicles: towards the development of vaccine and diagnostic candidate antigens (2020, PhD , Primary Advisor)
Characterisation of Necator americanus excretory/secretory products (2020, PhD , Secondary Advisor)
Schistosoma mansoni extracellular vesicles: immunobiology and vaccine efficacy (2020, PhD , Secondary Advisor)

Collaboration

The map shows research collaborations by institution from the past 7 years.
Note: Map points are indicative of the countries or states that institutions are associated with.