Nitrate-ammonium utilisation for the green alga species Desmodesmus armatus reared in laboratory batch culture conditions

Monoclonal 1.2 L batch cultures of the green microalga Desmodesmus armatus (R. Chod.) Hegev. (culture accession: NQAIF301, sourced from the North Queensland Algal Culturing and Identification Facility at James Cook University, Townsville, QLD) were reared in standard Bold Basal Medium (BBM). Cultures were kept in a temperature-controlled room at 27 ± 3°C with a 14-10 day-night cycle at a light intensity of 70 µmol photons m2 s-1. Nitrogen was set as the limiting factor for growth in all experimental cultures, supplied between 4 to 8 times below the recommended BBM concentration with either sodium nitrate (NaNO3) for nitrate-BBM, or ammonium chloride (NH4Cl) for ammonium-BBM, or both. Phosphorous, iron, and all other nutrients were supplied at non-limiting concentrations.

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    Data record related to this publication Nitrate-ammonium utilisation for the green alga species Desmodesmus armatus reared in laboratory batch culture conditions
    Data Publication title Nitrate-ammonium utilisation for the green alga species Desmodesmus armatus reared in laboratory batch culture conditions
  • Description

    Monoclonal 1.2 L batch cultures of the green microalga Desmodesmus armatus (R. Chod.) Hegev. (culture accession: NQAIF301, sourced from the North Queensland Algal Culturing and Identification Facility at James Cook University, Townsville, QLD) were reared in standard Bold Basal Medium (BBM). Cultures were kept in a temperature-controlled room at 27 ± 3°C with a 14-10 day-night cycle at a light intensity of 70 µmol photons m2 s-1. Nitrogen was set as the limiting factor for growth in all experimental cultures, supplied between 4 to 8 times below the recommended BBM concentration with either sodium nitrate (NaNO3) for nitrate-BBM, or ammonium chloride (NH4Cl) for ammonium-BBM, or both. Phosphorous, iron, and all other nutrients were supplied at non-limiting concentrations.

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  • Keywords
    • intraspecific trait variability
    • allometric scaling
    • process noise
    • observation error
    • nitrogen assimilation
    • kinetic parameters
    • process-based models
    • time-series
    • green alga
    • environmental gradients
    • ARC Centre of Excellence for Coral Reef Studies
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    Tropical Ecosystems, Conservation and Climate Change
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    Citation Malerba, Martino; Connolly, Sean; Heimann, Kirsten (2016): Nitrate-ammonium utilisation for the green alga species Desmodesmus armatus reared in laboratory batch culture conditions . James Cook University. https://doi.org/10.4225/28/56A1BD8A2258D