Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?

The following dataset was collected as part of a PhD thesis candidature by Alejandro Trujillo-González, enrolled in James Cook University, Townsville, Australia.

Data provides  threshold cycles (Ct) and melting temperature (Tm) values determined for amplicons in quantitative PCR tests and assays ran in a QuantStudio3™ Real-Time PCR System (ThermoFisher Scientific Inc., Brisbane) using QuantStudio™ Design and Analysis Software (version 1.4.2).

The full methodology is available in the publication shown in the Related Publications link below.

    Data Record Details
    Data record related to this publication Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?
    Data Publication title Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?
  • Description

    The following dataset was collected as part of a PhD thesis candidature by Alejandro Trujillo-González, enrolled in James Cook University, Townsville, Australia.

    Data provides  threshold cycles (Ct) and melting temperature (Tm) values determined for amplicons in quantitative PCR tests and assays ran in a QuantStudio3™ Real-Time PCR System (ThermoFisher Scientific Inc., Brisbane) using QuantStudio™ Design and Analysis Software (version 1.4.2).

    The full methodology is available in the publication shown in the Related Publications link below.

  • Other Descriptors
    • Descriptor

      This dataset is available as a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)

    • Descriptor type Note
  • Data type dataset
  • Keywords
    • environmental DNA
    • Neobenedenia girellae
    • biosecurity
    • border control
    • eDNA screening
  • Funding source
  • Research grant(s)/Scheme name(s)
    • - Australian Government through the Fisheries Research and Development Corporation (FRDC) (Project No. 2014/001), the University of Sydney, and James Cook University
  • Research themes
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    SEO Codes
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date 2017/05/15
  • End Date 2017/05/26
  • Time Period
    Spatial (location) coverage
  • Locations
    • Marine and Aquaculture Research Facility Unit (James Cook University, Australia)
    Data Locations

    Type Location Notes
    Attachment eDNA_validation_data_11092018.ods Open Document (.ods) format
    Attachment eDNA_validation_data_11092018.xlsx MS Excel (.xlsx) format
    The Data Manager is: Alejandro Trujillo Gonzalez
    College or Centre
    Access conditions Open: free access under license
  • Alternative access conditions
  • Data record size 2 files: 467 KB
  • Related publications
      Name Trujillo-Gonzalez, A., Becker, J. A., Huerlimann, R., Saunders, R. J., and Hutson, K. S. (2020) Can environmental DNA be used for aquatic biosecurity in the aquarium fish trade? Biological Invasions, 22, 1011-1025.
    • URL http://dx.doi.org/10.1007/s10530-019-02152-0
    • Notes
  • Related websites
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    • Notes
  • Related metadata (including standards, codebooks, vocabularies, thesauri, ontologies)
  • Related data
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    • Notes
    Citation Trujillo Gonzalez, Alejandro (2018): Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?. James Cook University. https://doi.org/10.25903/5bd295b16d2ad