Research Data

In vivo percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in Amphibian Ringer’s Solution

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General
Title
In vivo percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in Amphibian Ringer’s Solution
Type
Dataset
Date Record Created
2019-08-09
Date Record Modified
(no information)
Language
English
Coverage
Date Coverage
2018-10-18 to 2018-11-05
Time Period
(no information)
Geospatial Location
(no information)
Description
Descriptions
  1. Type: brief

    In vivo percutaneous absorption kinetics of caffeine, benzoic acid, and ibuprofen in the terrestrial toad Rhinella marina.

  2. Type: full

    Previous studies by the authors measured in vitro absorption kinetics of model chemicals through Rh. marina skin when the chemicals were formulated as saturated solutions in Amphibian Ringers Solution. However, while there is evidence that in vitro absorption kinetics provide relatively good correlation with the in vivo situation in mammals, it is unknown how well in vitro data matches the in vivo situation in frogs. This dataset provides in vivo absorption kinetics for three model chemicals - benzoic acid, caffeine, and ibuprofen – formulated in Amphibian Ringer’s solution, in the cane toad Rhinella marina. 

    Each chemical was formulated individually as a saturated solution (infinite dose) in Amphibian Ringer’s solution. 25 ml of the resultant solution was provided in a bath formulation to each animal. This volume ensured exposure of solution to the entire ventral pelvis in each animal.  Four animals were sacrificed at each sampling time and a cardiac blood sample taken. Following extraction of serum, chemical quantification was undertaken using UHPLC. Chemical flux was determined from the steady-state slope of the cumulative absorption vs time plot. 

    Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, sex, snout-vent length and weight), chemical information including name and concentration, and serum concentration of chemical when sampled.

    Column headings for the spreadsheet are explained below:

    Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen)

    Conc: concentration of chemical applied (microgram/ml)

    AnimalID: individual animal identifier

    Weight: frog weight in grams

    SVL: animal snout-vent length (millimetres)

    SA.total: total skin surface area calculated using [SAtotal=9.9*(animal weight^0.56)] (1)

    SA.pelvic:skin surface area of the pelvic ventrum, calculated using [SApelvic=1.15*(animal weight^0.559] (2)

    Time: sampling time (hours)

    Serum.conc: amount (micrograms) of chemical in 1 millilitre of serum (i.e., concentration in micrograms per millilitre)

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People
Creators
  1. Owned by: Mrs Victoria Llewelyn , victoria.llewelyn@my.jcu.edu.au , Pharmacy
Primary Contact
Mrs Victoria Llewelyn, victoria.llewelyn@my.jcu.edu.au
Supervisors
  1. Prof Beverley Glass , beverley.glass@jcu.edu.au
  2. Dr Lee Berger , lee.berger@jcu.edu.au
Collaborators
(no information)
Subject
Fields of Research
  1. 070710 - Veterinary Pharmacology (070710)
  2. 111504 - Pharmaceutical Sciences (111504)
Socio-Economic Objective
(no information)
Keywords
  1. Anuran
  2. skin absorption
  3. amphibian
Research Activity
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Research Themes
Tropical Ecosystems, Conservation and Climate Change
Tropical Health, Medicine and Biosecurity
Rights
License
CC BY-NC 4.0: Attribution-Noncommercial 4.0 International
License - Other
(no information)
Access Rights/Conditions
Please contact the data manager or nominated primary contact to negotiate access to this data. If you have difficulty, please contact researchdata@jcu.edu.au for assistance.
Type
conditional
Rights
(no information)
Data
Data Location
Online Locations
Stored At
Secure data section of the Tropical Data Hub (TDH) archive - eResearch Centre, James Cook University, Townsville, Queensland, Australia
Citation
Cite:
Llewelyn, V. (2019): In vivo percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in Amphibian Ringer’s Solution. James Cook University. (dataset). http://dx.doi.org/10.25903/5d4d00bb0d310
Digital Object Identifier (DOI):
10.25903/5d4d00bb0d310