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Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva

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General
Title
Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva
Type
Dataset
Date Record Created
2019-08-16
Date Record Modified
(no information)
Language
English
Coverage
Date Coverage
2017 to 2019
Time Period
(no information)
Geospatial Location
Description
Descriptions
  1. Type: full

    Abstract [Related Publication]: Malaria is the most important vector-borne disease in the world. Epidemiological and ecological studies of malaria traditionally utilize detection of Plasmodium sporozoites in whole mosquitoes or salivary glands by microscopy or serological or molecular assays. However, these methods are labor-intensive, and can over- or underestimate mosquito transmission potential. To overcome these limitations, alternative sample types have been evaluated for the study of malaria. It was recently shown that Plasmodium could be detected in saliva expectorated on honey-soaked cards by Anopheles stephensi, providing a better estimate of transmission risk. We evaluated whether excretion of Plasmodium falciparum nucleic acid by An. stephensi correlates with expectoration of parasites in saliva, thus providing an additional sample type for estimating transmission potential. Mosquitoes were exposed to infectious blood meals containing cultured gametocytes, and excreta collected at different time points post-exposure. Saliva was collected on honey-soaked filter paper cards, and salivary glands were dissected and examined microscopically for sporozoites. Excreta and saliva samples were tested by real time polymerase chain reaction (RT-rtPCR).

    The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

    • Description (parameters)
    • Plasmodium in excreta from groups of mosquitoes
    • Plasmodium in excreta, saliva and salivary glands 
    • Evaluation of parasite development in the mosquito - Parasite_dev_oocyst
    • Evaluation of parasite development in the mosquito - Parasite_dev_sporos
    • Survival analysis

    The full methodology is available in the Open Access publication from the Related Publications link below.

     

  2. Type: note

    This dataset is available as a spreadsheet saved in both MS Excel (.xlsx) and Open Document (.ods) formats.

Related Publications
  1. Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes
    Open Access
Related Websites
(no information)
Related Data
(no information)
Related Services
(no information)
Technical metadata
(no information)
People
Creators
  1. Managed by: Miss Ana Ramirez Lopez , ana.ramirez1@my.jcu.edu.au , College of Public Health, Medical & Vet Sciences, Australian Institute of Tropical Health & Medicine
Primary Contact
Miss Ana Ramirez Lopez, ana.ramirez1@my.jcu.edu.au
Supervisors
  1. Prof Scott Ritchie , scott.ritchie@jcu.edu.au
Collaborators
  1. Andrew F. van den Hurk, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland
  2. Ian M. Mackay, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government
  3. Annie S. P. Yang, Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research
  4. Glen R. Hewitson, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government
  5. Jamie L. McMahon, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government
  6. Justin A. Boddey, Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research
  7. Sara M. Erickson, Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research
Subject
Fields of Research
  1. 060808 - Invertebrate Biology (060808)
  2. 060599 - Microbiology not elsewhere classified (060599)
  3. 119999 - Medical and Health Sciences not elsewhere classified (119999)
Socio-Economic Objective
  1. 92 - Health (92)
Keywords
  1. mosquito
  2. excreta
  3. malaria
  4. saliva
  5. sporozoite
  6. Plasmodium falciparum
  7. Anopheles stephensi
Research Activity
(no information)
Research Themes
Tropical Health, Medicine and Biosecurity
Rights
License
CC BY-NC-ND: Attribution-Noncommercial-No Derivatives 3.0 AU
License - Other
(no information)
Access Rights/Conditions
Open access. If the data is not freely accessible via the link provided, please contact the nominated data manager or researchdata@jcu.edu.au for assistance.
Type
open
Rights
(no information)
Data
Data Location
Online Locations
Attachments
  1. Data_Plasmodium_mosquito_excreta_saliva.xlsx (Data File, Public)
  2. Data_Plasmodium_mosquito_excreta_saliva.ods (Data File, Public)
Stored At
(no information)
Citation
Cite:
Ramirez Lopez, A. (2019): Dataset describing the detection of Plasmodium falciparum in mosquito excreta and saliva. James Cook University. (dataset). http://dx.doi.org/10.25903/5d5de1e49aae9
Digital Object Identifier (DOI):
10.25903/5d5de1e49aae9