Research Data

Dataset describing stability of WNV RNA in mosquito excreta

Details

Print this Page Print this Page
Show all sections
General
Title
Dataset describing stability of WNV RNA in mosquito excreta
Type
Dataset
Date Record Created
2019-08-16
Date Record Modified
2019-08-23
Language
English
Coverage
Date Coverage
2018 to 2019
Time Period
(no information)
Geospatial Location
Description
Descriptions
  1. Type: full

     Abstract [Related Publication]: Arbovirus surveillance is crucial for the implementation of vector-borne disease control measures. Recently, it has been demonstrated that mosquitoes with a disseminated arbovirus infection excrete viral RNA, which can be detected by molecular methods. Thereby, mosquito excreta has been proposed as a sample type that could be utilized for arbovirus surveillance. In this study, we evaluated if West Nile virus (Kunjin strain, WNVKUN) RNA in Culex annulirostris Skuse (Diptera: Culicidae) excreta deposited on different substrates could be detected after storage for up to 2 wk at tropical conditions of high heat and humidity. No significant drop in relative quantity of WNVKUN RNA (determined by comparison of Ct values) in excreta deposited on Flinders Associate Technologies (FTA) cards was observed over 14 d, suggesting that RNA was stable for that time. There was no significant difference in relative quantity of WNVKUN RNA in excreta deposited on FTA cards or polycarbonate substrates after 24 h. However, after 7 and 14 d, there was a significant decline in the relative quantity of viral RNA in the excreta stored on polycarbonate substrates. For incorporation in arbovirus surveillance programs, we recommend the use of polycarbonate substrates for excreta collection in mosquito traps deployed overnight, and the integration of FTA cards in traps serviced weekly or fortnightly. Polycarbonate substrates facilitate the collection of the majority of excreta from a trap, and while FTA cards offer limited area coverage, they enable preservation of viral RNA in tropical conditions for extended periods of time.

    The dataset consists of a 6 worksheets and includes a description of the parameters for each of the 5 experiments listed below and the data for each experiment:

    • RRV-groups
    • WNV-groups
    • RRV-individuals
    • WNV-individuals
    • Viability of viral RNA in mosquito excreta

     The full methodology is available in the publication shown in the Related Publications link below.

  2. Type: note

    This dataset is available as a spreadsheet saved in both MS Excel (.xlsx) and Open Document (.ods) formats.

Related Publications
  1. Ramírez, Ana L., Hall-Mendelin, Sonja, Hewitson, Glen R., McMahon, Jamie L., Staunton, Kyran M., Ritchie, Scott A., and van den Hurk, Andrew F. (2019) Stability of West Nile Virus (Flaviviridae: Flavivirus) RNA in mosquito excreta. Journal of Medical Entomology, 56 (4). pp. 1135-1138.
Related Websites
(no information)
Related Data
(no information)
Related Services
(no information)
Technical metadata
(no information)
People
Creators
  1. Managed by: Miss Ana Ramirez Lopez , ana.ramirez1@my.jcu.edu.au , College of Public Health, Medical & Vet Sciences, Australian Institute of Tropical Health & Medicine
Primary Contact
MS Ana Ramirez Lopez, ana.ramirez1@my.jcu.edu.au
Supervisors
  1. Prof Scott Ritchie , scott.ritchie@jcu.edu.au
Collaborators
  1. Sonja Hall-Mendelin, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland, Australia
  2. Glen R. Hewitson, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland, Australia
  3. Jamie L. McMahon, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland, Australia
  4. Kyran M. Staunton, College of Public Health, Medical and Veterinary Sciences, James Cook University
  5. Andrew F. van den Hurk, Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland, Australia
Subject
Fields of Research
  1. 069999 - Biological Sciences not elsewhere classified (069999)
  2. 119999 - Medical and Health Sciences not elsewhere classified (119999)
Socio-Economic Objective
  1. 929999 - Health not elsewhere classified (929999)
Keywords
  1. mosquito
  2. arbovirus
  3. excreta
  4. RNA stability
  5. surveillance
Research Activity
(no information)
Research Themes
Tropical Health, Medicine and Biosecurity
Rights
License
CC BY-NC-ND: Attribution-Noncommercial-No Derivatives 3.0 AU
License - Other
(no information)
Access Rights/Conditions
Open access. If the data is not freely accessible via the link provided, please contact the nominated data manager or researchdata@jcu.edu.au for assistance.
Type
open
Rights
(no information)
Data
Data Location
Online Locations
Attachments
  1. Data_RNA_stability_WNV_mosquito_excreta.xlsx (Data File, Public)
  2. Data_RNA_stability_WNV_mosquito_excreta.ods (Data File, Public)
Stored At
(no information)
Citation
Cite:
Ramirez Lopez, A. (2019): Dataset describing stability of WNV RNA in mosquito excreta. James Cook University. (dataset). http://dx.doi.org/10.25903/5d5f2a38e823f
Digital Object Identifier (DOI):
10.25903/5d5f2a38e823f