
- Title
- Potential allergens of the Pacific Oyster (Crassostrea gigas)
- Type
- Dataset
- Date Record Created
- 2017-01-30
- Date Record Modified
- 2017-03-24
- Language
- English
- Date Coverage
- (no information)
- Time Period
- (no information)
- Geospatial Location
- (no information)
- Descriptions
-
- Type: full
The increasing production and consumption of molluscs are associated with a rise in the prevalence of mollusc allergy worldwide, currently affecting 0.2% to 1.3% of the general population. However, the elucidation of mollusc allergens, crucial for better diagnostics, still lags behind other seafood groups such as fish and crustacean. Genomic data have previously been utilized for the improved identification of non-food allergens by performing similarity searches using the BLAST program. Based on the published genome of the Pacific oyster (Crassostrea gigas) we aimed to identify the repertoire of potential allergen using bioinformatics analysis and sought to validate allergenicity using a combination of immuno-chemical methods and proteomic analysis. A repertoire of 25,982 genome-derived proteomes of the Pacific oyster were aligned with 2117 allergen sequences resulting in over 800 protein homologues. Of those, 95 proteins were potentially cross-reactive allergens due to high identity with known allergens (>50% identity). Analysis of the transcriptomic data showed the proteins were differentially expressed across tissue of the oyster.
- Type: note
This dataset consists of a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)
- Type: full
- Related Publications
- Related Websites
- (no information)
- Related Data
- (no information)
- Related Services
- (no information)
- Technical metadata
- (no information)
- Creators
-
- Managed by: Prof Andreas Lopata , andreas.lopata@jcu.edu.au , Discipline of Biochemistry & Molecular Biology (6272), Centre for Biodiscovery and Molecular Development of Therapeutics, Centre for Sustainable Tropical Fisheries and Aquaculture
- Managed by: Dr Sandip Kamath , sandip.kamath@jcu.edu.au , Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Medicine (6205), Discipline of Biochemistry & Molecular Biology (6272)
- Aggregated by: Mr Roni Nugraha , roni.nugraha@my.jcu.edu.au , Discipline of Biochemistry & Molecular Biology (6272), Centre for Tropical Biodiversity and Climate Change
- Primary Contact
- A/Prof Andreas Lopata, andreas.lopata@jcu.edu.au
- Supervisors
-
- Prof Andreas Lopata , andreas.lopata@jcu.edu.au
- Dr Sandip Kamath , sandip.kamath@jcu.edu.au
- Dr Kyall Zenger , kyall.zenger@jcu.edu.au
- Collaborators
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- Prof. Jennifer M Rolland, Department of Immunology and Pathology, Monash University, Melbourne, Victoria, Australia
- Prof. Robyn E. O'Hehir, Department of Immunology and Pathology, Monash University, Melbourne, Victoria, Australia
- Fields of Research
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- 110701 - Allergy (110701)
- 110106 - Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics) (110106)
- Socio-Economic Objective
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- 920108 - Immune System and Allergy (920108)
- 920203 - Diagnostic Methods (920203)
- Keywords
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- food allergens
- oyster
- genomic
- in-silico
- immunoinformatics
- Research Activity
- (no information)
- Research Themes
- Industries and Economies in the Tropics
- Tropical Health, Medicine and Biosecurity
- License
- CC BY-NC: Attribution-Noncommercial 3.0 AU
- License - Other
- (no information)
- Access Rights/Conditions
- Please contact the data manager or nominated primary contact to negotiate access to this data. If you have difficulty, please contact researchdata@jcu.edu.au for assistance.
- Type
- conditional
- Rights
- Once access to the data has been obtained via negotiation with the data manager, use of the dataset is governed by the CC-BY-NC AU licence.
- Data Location
- Online Locations
- Stored At
- Secure data section of the Tropical Data Hub (TDH) archive - eResearch Centre, James Cook University, Townsville, Queensland, Australia
- Cite:
- Lopata, A.; Kamath, S.; Nugraha, R. (2017). Potential allergens of the Pacific Oyster (Crassostrea gigas). James Cook University. [Data Files] http://dx.doi.org/10.4225/28/588ebc2ca00e2
- Digital Object Identifier (DOI):
- 10.4225/28/588ebc2ca00e2