Research Data

Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?

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General
Title
Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?
Type
Dataset
Date Record Created
2018-09-11
Date Record Modified
2020-03-02
Language
English
Coverage
Date Coverage
2017-05-15 to 2017-05-26
Time Period
(no information)
Geospatial Location
  • Marine and Aquaculture Research Facility Unit (James Cook University, Australia)
Description
Descriptions
  1. Type: brief

    The following dataset was collected as part of a PhD thesis candidature by Alejandro Trujillo-González, enrolled in James Cook University, Townsville, Australia.

    Data provides  threshold cycles (Ct) and melting temperature (Tm) values determined for amplicons in quantitative PCR tests and assays ran in a QuantStudio3™ Real-Time PCR System (ThermoFisher Scientific Inc., Brisbane) using QuantStudio™ Design and Analysis Software (version 1.4.2).

    The full methodology is available in the publication shown in the Related Publications link below.

  2. Type: note

    This dataset is available as a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)

Related Publications
  1. Trujillo-Gonzalez, A., Becker, J. A., Huerlimann, R., Saunders, R. J., and Hutson, K. S. (2020) Can environmental DNA be used for aquatic biosecurity in the aquarium fish trade? Biological Invasions, 22, 1011-1025.
Related Websites
(no information)
Related Data
(no information)
Related Services
(no information)
Technical metadata
(no information)
People
Creators
  1. Owned by: Mr Alejandro Trujillo Gonzalez , alejandro.trujillogonzalez@my.jcu.edu.au , College of Science & Engineering, Centre for Sustainable Tropical Fisheries and Aquaculture
Primary Contact
Mr Alejandro Trujillo Gonzalez, alejandro.trujillogonzalez@my.jcu.edu.au
Supervisors
  1. Dr Kate Hutson , kate.hutson@jcu.edu.au
Collaborators
  1. Dr Richard J. Saunders, 3. Animal Science, Queensland Department of Agriculture and Fisheries
  2. Dr Joy A. Becker, 2. School of Life and Environmental Sciences, Faculty of Science, University of Sydney, 425 Werombi road, Camden, NSW 2570, Australia.
Subject
Fields of Research
  1. 050199 - Ecological Applications not elsewhere classified (050199)
  2. 060802 - Animal Cell and Molecular Biology (060802)
Socio-Economic Objective
  1. 830102 - Aquaculture Fin Fish (excl. Tuna) (830102)
  2. 910302 - Trade Assistance and Protection (910302)
Keywords
  1. environmental DNA
  2. Neobenedenia girellae
  3. biosecurity
  4. border control
  5. eDNA screening
Research Activity
(no information)
Research Themes
Tropical Health, Medicine and Biosecurity
Rights
License
CC BY-NC-ND: Attribution-Noncommercial-No Derivatives 3.0 AU
License - Other
(no information)
Access Rights/Conditions
Open access. If the data is not freely accessible via the link provided, please contact the nominated data manager or researchdata@jcu.edu.au for assistance.
Type
open
Rights
(no information)
Data
Data Location
Online Locations
Attachments
  1. eDNA_validation_data_11092018.ods (Data File, Public)
  2. eDNA_validation_data_11092018.xlsx (Data File, Public)
Stored At
(no information)
Citation
Cite:
Trujillo Gonzalez, A. (2018). Can environmental DNA be used to reliably detect parasites in the aquarium fish trade?. James Cook University. (dataset). http://dx.doi.org/10.25903/5bd295b16d2ad
Digital Object Identifier (DOI):
10.25903/5bd295b16d2ad