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Parasite detection in the ornamental fish trade using environmental DNA

The following dataset was collected as part of a PhD thesis candidature by Alejandro Trujillo-González. Data provides threshold cycles (Ct) and melting temperature (Tm) values determined for amplicons in quantitative PCR tests and assays ran in a QuantStudio3™ Real-Time PCR System (ThermoFisher Scientific Inc., Brisbane) using QuantStudio™ Design and Analysis Software (version 1.4.2).

The full methodology is available in the Open Access publication from the Related Publications link below.
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    Data Record Details
    Data record related to this publication Parasite detection in the ornamental fish trade using environmental DNA
    Data Publication title Parasite detection in the ornamental fish trade using environmental DNA
  • Description

    The following dataset was collected as part of a PhD thesis candidature by Alejandro Trujillo-González. Data provides threshold cycles (Ct) and melting temperature (Tm) values determined for amplicons in quantitative PCR tests and assays ran in a QuantStudio3™ Real-Time PCR System (ThermoFisher Scientific Inc., Brisbane) using QuantStudio™ Design and Analysis Software (version 1.4.2).

    The full methodology is available in the Open Access publication from the Related Publications link below.

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  • Other Descriptors
    • Descriptor

      This dataset is available as a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)

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      Descriptor type Note
    • Data type dataset
  • Keywords
    • environmental DNA
    • ornamental fish
    • border control
    • Dactylogyrus
    • quantitative PCR
  • Funding source
  • Research grant(s)/Scheme name(s)
    • 21890 - (James Cook University Research Activities) Aquatic animal health subprogram: Strategic approaches to identifying pathogens of quarantine concern associated with the importation of ornamental fish
    • Research themes
    Tropical Ecosystems, Conservation and Climate Change
    Tropical Health, Medicine and Biosecurity
    FoR Codes (*)
    SEO Codes
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    Temporal (time) coverage
  • Start Date 2015/01/12
  • End Date 2015/10/31
    • Time Period
    Spatial (location) coverage
  • Locations
    • Following release by Quarantine Services (Department of Agriculture and Water Resources), fish were transported to an Approved Arrangement Laboratory at the Sydney School of Veterinary Sciences, University of Sydney, Camden, Australia
- Data
    Data Locations

    Type Location Notes
    Attachment Dataset_Parasite_Detection.ods Open Document (.ods) format
    Attachment Dataset_Parasite_Detection.xlsx MS Excel (.xlsx) format
    The Data Manager is: Alejandro Trujillo Gonzalez
    College or Centre
    Access conditions Open: free access under license
  • Alternative access conditions
    • Data record size 2 files: 98.1 KB
- Related resources
  • Related publications
      Name Trujillo-González, Alejandro, Edmunds, R. C., Becker, J. A., and Hutson, K. S. (2019) Parasite detection in the ornamental fish trade using environmental DNA. Scientific Reports, 9. 5173.
    • URL https://doi.org/10.1038/s41598-019-41517-2
    • Notes Open Access
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- License
- Citation
    Citation Trujillo Gonzalez, Alejandro (2018): Parasite detection in the ornamental fish trade using environmental DNA. James Cook University. https://doi.org/10.25903/5b90c1897397a