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Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals

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General
Title
Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals
Type
Dataset
Date Record Created
2017-11-30
Date Record Modified
2018-01-02
Language
English
Coverage
Date Coverage
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Time Period
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Geospatial Location
  • Adult colonies collected from the reef adjacent to Orpheus Island on the northern Great Barrier Reef (18.60°S, 146.49°E) and transported to Orpheus Island Research Station
Description
Descriptions
  1. Type: full

    Acropora millepora transcriptome from the National Center for Biotechnology Information, BioProject PRJNA288809. Submitted by ARC Centre of Excellence for Coral Reef Studies, James Cook University.

    Experiment type: Expression profiling by high throughput sequencing.

    Summary: The metabolic bases of the interaction between the coral Acropora millepora and its dinoflagellate symbiont were investigated by comparing gene expression levels under light and dark conditions at the whole transcriptome level. Among the differentially expressed genes identified, a suite of genes involved in cholesterol transport was found to be up-regulated under light conditions, confirming the significance of this compound in the coral symbiosis. Although ion transporters likely to have roles in calcification were not differentially expressed in this study, expression levels of many genes associated with skeletal organic matrix composition and organization were higher in light conditions. This implies that the rate of organic matrix synthesis is one factor limiting calcification at night. Thus, LEC during the day is likely to be a consequence of increases in both matrix synthesis and the supply of precursor molecules as a result of photosynthetic activity.

    Overall design:  Branch tips from three adult colonies of Acropora millepora were sampled at midday and midnight.

    Abstract [Related Publication]: The evolutionary success of reef-building corals is often attributed to their symbiotic relationship with photosynthetic dinoflagellates of the genus Symbiodinium, but metabolic interactions between the partners and the molecular bases of light-enhanced calcification (LEC) are not well understood. Here, the metabolic bases of the interaction between the coral Acropora millepora and its dinoflagellate symbiont were investigated by comparing gene expression levels under light and dark conditions at the whole transcriptome level. Among the 497 differentially expressed genes identified, a suite of genes involved in cholesterol transport was found to be upregulated under light conditions, confirming the significance of this compound in the coral symbiosis. Although ion transporters likely to have roles in calcification were not differentially expressed in this study, expression levels of many genes associated with skeletal organic matrix composition and organization were higher in light conditions. This implies that the rate of organic matrix synthesis is one factor limiting calcification at night. Thus, LEC during the day is likely to be a consequence of increases in both matrix synthesis and the supply of precursor molecules as a result of photosynthetic activity.

     

     

Related Publications
  1. Bertucci, A., Forêt, S., Ball, E.E., and Miller, D.J. (2015) Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals. Molecular Ecology, 24 (17). pp. 4489-4504.
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Related Data
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Technical metadata
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People
Creators
  1. Managed by: Dr Anthony Bertucci , anthony.bertucci@jcu.edu.au , ARC Centre of Excellence for Coral Reef Studies (7351)
  2. Associated with: Dr Sylvain Foret , ARC Centre of Excellence for Coral Reef Studies
  3. Associated with: Prof David Miller , david.miller@jcu.edu.au , ARC Centre of Excellence for Coral Reef Studies, Molecular and Cell Biology, Comparative Genomics Centre
Primary Contact
Dr Anthony Bertucci, anthony.bertucci@jcu.edu.au
Supervisors
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Collaborators
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Subject
Fields of Research
  1. 060409 - Molecular Evolution (060409)
Socio-Economic Objective
(no information)
Keywords
  1. transcriptome
  2. Symbiodinium
  3. gene expression
  4. biomineralization
  5. cholesterol
  6. light-enhanced calcification
  7. organic matrix
  8. symbiosis
  9. ARC Centre of Excellence for Coral Reef Studies
Research Activity
(no information)
Research Themes
Tropical Ecosystems, Conservation and Climate Change
Rights
License
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License - Other
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Access Rights/Conditions
NCBI places no restrictions on the use or distribution of data.
Type
open
Rights
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Data
Data Location
Online Locations
  1. https://www.ncbi.nlm.nih.gov/bioproject/288809
Stored At
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Citation
Cite:
Bertucci, A.; Foret, S.; Miller, D. (2015). Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals. James Cook University. (dataset). 79d927c0616666f4c1ee2bf7ef7312cf