Research Data

Tilapia eDNA (environmental DNA) data

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General
Title
Tilapia eDNA (environmental DNA) data
Type
Dataset
Date Record Created
2017-03-22
Date Record Modified
2017-11-23
Language
English
Coverage
Date Coverage
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Time Period
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Geospatial Location
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Description
Descriptions
  1. Type: full

    Environmental DNA data from Robson HLA, Noble TH, Saunders RJ, Robson SKA, Burrows DW, Jerry DR (2016) Fine tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems.

    Data consists of 4 files:

    (1) Presence/absence data for filtration experiment 1 using 3, 10 and 20 um filters, with and without a prefiltering step

    (2) Initial tilapia eDNA concentrations in ng at the start of Exp 2 on degradation for all three treatments, 23, 29 and 35 degrees Celcius

    (3) Presence/absence data for Exp 2 on degradation of tilapia eDNA for 3 treatments of temperature 23,29 and 35 degrees Celcius

    (4) Presence/absence data recorded as probability of detection for all qPCR replicates by day for all treatment pond densities (0,1,2,4,8,16,32, and up to 64 fish) for Experiment 3-Accumulation of tilapia eDNA.

    Extract from Abstract [Related publication]: Invasive species pose a major threat to aquatic ecosystems. Their impact can be particularly severe in tropical regions, like those in northern Australia, where 20 invasive fish species are recorded. In temperate regions, environmental DNA (eDNA) technology is gaining momentum as a tool to detect aquatic pests, but the technology's effectiveness has not been fully explored in tropical systems with their unique climatic challenges (i.e. high turbidity, temperatures and ultraviolet light). In this study, we modified conventional eDNA protocols for use in tropical environments using the invasive fish, Mozambique tilapia (Oreochromis mossambicus) as a detection model. We evaluated the effects of high water temperatures and fish density on the detection of tilapia eDNA, using filters with larger pores to facilitate filtration.

    The full methodology is available in the publication shown in the Related Publications link below.

  2. Type: note

    This dataset is available from Dryad in MS Excel (.xls) format. Dryad data package: Robson HLA, Noble TH, Saunders RJ, Robson SKA, Burrows DW, Jerry DR (2016) Data from: Fine tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.6n5s3

Related Publications
  1. Robson, Heather L.A., Noble, Tansyn H., Saunders, Richard J., Robson, Simon K.A., Burrows, Damien W., and Jerry, Dean R. (2016) Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems. Molecular Ecology, 16 (4). pp. 922-932.
Related Websites
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Related Data
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Related Services
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Technical metadata
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People
Creators
  1. Associated with: Mrs Heather Robson , heather.robson@jcu.edu.au , Terrestrial Ecosystems & Climate Change (223400), Zoology and Ecology
  2. Associated with: Miss Tansyn Noble , tansyn.noble@jcu.edu.au , Marine Biology & Aquaculture
  3. Associated with: Dr Richard Saunders , richard.saunders@jcu.edu.au , Aquaculture & Fisheries (223200), Division of Tropical Environments & Societies, Centre for Sustainable Tropical Fisheries and Aquaculture
  4. Prof Simon Robson , simon.robson@jcu.edu.au , Terrestrial Ecosystems & Climate Change (223400), Centre for Tropical Biodiversity and Climate Change
  5. Prof Damien Burrows , damien.burrows@jcu.edu.au , Tropical Water & Ecosystems (TropWater), Centre for Tropical Water and Aquatic Ecosystem Research
  6. Prof Dean Jerry , dean.jerry@jcu.edu.au , Aquaculture & Fisheries (223200), Centre for Tropical Water and Aquatic Ecosystem Research, Centre for Sustainable Tropical Fisheries and Aquaculture
Primary Contact
Prof Simon Robson, simon.robson@jcu.edu.au
Supervisors
(no information)
Collaborators
(no information)
Subject
Fields of Research
  1. 060204 - Freshwater Ecology (060204)
  2. 070401 - Aquaculture (070401)
Socio-Economic Objective
  1. 960506 - Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments (960506)
Keywords
  1. environmental DNA
  2. eDNA filtration
  3. cichlid
  4. Oreochromis mossambicus
  5. Mozambique tilapia
  6. invasive fish species
Research Activity
(no information)
Research Themes
Tropical Ecosystems, Conservation and Climate Change
Rights
License
(no information)
License - Other
CC 0: Public Domain Dedication 1.0 Universal
Access Rights/Conditions
Open access. If the data is not freely accessible via the link provided, please contact the nominated data manager or researchdata@jcu.edu.au for assistance.
Type
open
Rights
(no information)
Data
Data Location
Online Locations
  1. http://dx.doi.org/10.5061/dryad.6n5s3
Stored At
(no information)
Citation
Cite:
Robson, H.; Noble, T.; Saunders, R.; Robson, S.; Burrows, D.; Jerry, D. (2016). Tilapia eDNA (environmental DNA) data. James Cook University. [Data Files] 7c10f9cbc3ee296684e57a4441861153